C3934
碳酸酐酶 来源于牛红细胞
lyophilized powder, ≥2,000 W-A units/mg protein
别名:
碳酸盐脱水酶, 碳酸酯水解酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-576-6
MDL number:
Specific activity:
≥2,000 W-A units/mg protein
Biological source:
bovine erythrocytes
Application
牛红血球(BCA)来源碳酸酐酶已用于研究去除酶结合金属离子Zn2+对酶的聚集行为的影响。使用螯合剂（例如EDTA（乙二胺四乙酸） ）去除金属离子，增强了酶采用熔融球状态的倾向。人们发现，这种状态的酶可与分子伴侣样α-晶状体蛋白结合并防止聚集。来自Sigma的酶已固定在电化学传感器上，以便开发出彻底溶解CO2的分析装置。其还用于BCA构象变化的热力学分析。
Biochem/physiol Actions
碳酸酐酶是一种锌金属酶，其分子量约为30,000Da。该酶催化二氧化碳水合成碳酸。它参与重要的生理和病理过程，如pH和CO2稳态、碳酸氢盐和CO2的转运、生物合成反应、骨吸收、钙化和致肿瘤性。因此，该酶是用于各种病理学（如青光眼、癫痫和帕金森病）的临床应用的抑制剂的重要靶标。
Other Notes
在0°C条件下，一Wilbur-Anderson (W-A)单位可使0.02M Trizma缓冲液pH在一分钟内从8.3降至 6.3。（一W-A单位基本上相当于一Roughton-Booth单位。）
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
动植物源性产品
此项目有
Fraser J Moss et al.
The Journal of physiology, 598(24), 5821-5856 (2020-09-25)
According to the HCO 3 - metabolon hypothesis, direct association of cytosolic carbonic anhydrases (CAs) with the electrogenic Na/HCO3 cotransporter NBCe1-A speeds transport by regenerating/consuming HCO 3 - . The present work addresses published discrepancies as to whether
Use of carbonic anhydrase in electrochemical biosensors for dissolved CO2
Cammaroto C, et al.
Sensors and Actuators B, Chemical, 48(1-3), 439-447 (1998)
Mikhail Krasavin et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 165-171 (2019-11-23)
Testing of an expanded, 800-compound set of analogues of the earlier described Strecker-type α-aminonitriles (selected from publicly available Enamine Ltd. Screening Collection) in thermal shift assay against bovine carbonic anhydrase (bCA) led to further validation of this new class of
Mikhail Krasavin et al.
Journal of enzyme inhibition and medicinal chemistry, 35(1), 306-310 (2019-12-05)
The differential scanning fluorimetry (DSF) screening of 5.692 fragments in combination with benzenesulfonamide (BSA) against bovine carbonic anhydrase (bCA) delivered >100 hits that either caused, on their own, a significant thermal shift (ΔTm, °C) in the protein melting temperature or
D Sorigué et al.
Science (New York, N.Y.), 372(6538) (2021-04-10)
Fatty acid photodecarboxylase (FAP) is a photoenzyme with potential green chemistry applications. By combining static, time-resolved, and cryotrapping spectroscopy and crystallography as well as computation, we characterized Chlorella variabilis FAP reaction intermediates on time scales from subpicoseconds to milliseconds. High-resolution
实验方案
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C3934-100MG | 04061833486665 |
| C3934-500MG | 04061833486696 |
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