应用
来自人红细胞（HCA）的碳酸酐酶已被用于研究碳酸酐酶（CA）的熔球态。分子伴侣样α-晶状体蛋白能够与这种状态的酶结合并阻止其聚集。 Sigma的酶已被用于分析酶的热力学稳定性。 此外，已经评估了该酶在人非小细胞肺癌中的临床意义。
生化/生理作用
碳酸酐酶是一种锌金属酶，其分子量约为30,000Da。该酶可以催化二氧化碳的水合，形成碳酸。它参与一些重要过程，如pH和CO2稳态、碳酸氢盐和CO2的转运、生物合成反应、骨吸收、钙化和致瘤性。因此，该酶是一个重要的抑制剂靶标，在青光眼、癫痫和帕金森氏病等各种病症中具有临床应用价值。
制备说明
色谱
纯化的。
纯化的。
其他说明
在0 °C条件下，一Wilbur-Anderson (W-A)单位可使0.02M Trizma缓冲液pH在一分钟内从8.3降至 6.3。（一W-A单位基本上相当于一Roughton-Booth单位。）
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--人源产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
K Rajaraman et al.
The Journal of biological chemistry, 271(44), 27595-27600 (1996-11-01)
alpha-Crystallin, a multimeric protein, exhibits chaperone-like activity in preventing aggregation of several proteins. We have studied the chaperone-like activity of alpha-crystallin toward heat-induced aggregation of bovine and human carbonic anhydrase. Human carbonic anhydrase aggregates at 60 degrees C, while bovine
Whei Ling Chiang et al.
Cancer letters, 188(1-2), 199-205 (2002-10-31)
This study was designed to elucidate the possible relationship between the expression of cytosolic carbonic anhydrase (CA) and non-small cell lung cancer (NSCLC). The activity and protein expression patterns of carbonic anhydrase I (CAI) and II (CAII) of 70 NSCLC
Daumantas Matulis et al.
Biochemistry, 44(13), 5258-5266 (2005-03-30)
ThermoFluor (a miniaturized high-throughput protein stability assay) was used to analyze the linkage between protein thermal stability and ligand binding. Equilibrium binding ligands increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. Binding
Melissa Pynnonen et al.
PLoS pathogens, 7(7), e1002104-e1002104 (2011-07-14)
Staphylococcus aureus nasal colonization is an important risk factor for community and nosocomial infection. Despite the importance of S. aureus to human health, molecular mechanisms and host factors influencing nasal colonization are not well understood. To identify host factors contributing
Pavel V Shliaha et al.
Journal of proteome research, 12(6), 2323-2339 (2013-03-22)
qTOF mass spectrometry and traveling wave ion mobility separation (TWIMS) hybrid instruments (q-TWIMS-TOF) have recently become commercially available. Ion mobility separation allows an additional dimension of precursor separation inside the instrument, without incurring an increase in instrument time. We comprehensively
实验方案
Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)
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