BID, Caspase-8-cleaved from mouse

Mouse BID cleaved with caspase-8 generates the amino-terminal fragment (amino acids 1-59, 7 kDa) and the carboxy-terminal fragment (amino acids 60-195, 15 kDa). On size exclusion chromatography cleaved BID elutes at 27 kDa indicating that the fragments remain associated.

Caspase-8-cleaved BID relocates from the cytosol to the outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability.

0.2 μm filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl.

Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.