recombinant
expressed in E. coli
Quality Segment
assay
≥95% (SDS-PAGE)
form
buffered aqueous solution
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Bid(12122)
General description
Mouse BID cleaved with caspase-8 generates the amino-terminal fragment (amino acids 1-59, 7 kDa) and the carboxy-terminal fragment (amino acids 60-195, 15 kDa). On size exclusion chromatography cleaved BID elutes at 27 kDa indicating that the fragments remain associated.
Biochem/physiol Actions
Caspase-8-cleaved BID relocates from the cytosol to the outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability.
Physical form
0.2 μm filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl.
Analysis Note
Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.
存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable