细胞色素P450 人

Human cytochrome P450 has been used in a study to investigate non-viral environmental risk factors for nasopharyngeal carcinoma. Human cytochrome P450 has also been used in a study to investigate the dynamics and hydration of the active sites of mammalian cytochromes.

细胞色素P450是异质性同工酶家族，其主要功能是氧化小分子，发挥中间代谢（如脂肪酸）功能和对外源性化合物（药物或毒素）进行解毒。有些同工型具有有限的底物特异性，而其他同工酶则比较混杂。CYP1A1亚型催化乙氧基试卤灵的7-脱乙基。细胞色素P450(CYP)在外源物解毒、细胞代谢和稳态中起重要作用。药物相互作用的一种主要机制就是激活或抑制这类酶。CYP酶可被多种外源物和内源底物经由受体依赖途径转录激活。基于代谢的药物相互作用主要影响就是抑制这类酶，许多化疗药物可通过抑制或诱导细胞色素P450酶系引发药物互作。

Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. This product has a molecular mass of approximately 58 kDa. Amiodarone, furafylline, and cimetidine are inhibitors, whereas tobacco, insulin, and omeprazole are inducers of the enzyme. The isozyme CYP1A2 is a major pathway for the 6-hydroxylation of melatonin in vivo. This isozyme also catalyzes the 5-hydroxylation of thiabendazole.

Microsomes containing recombinant human CYP1A2, recombinant rabbit NADPH-P450 reductase, and cytochrome b₅.

Solution in 100 mM potassium phosphate buffer_, pH 7.4.