Merck
CN

C5670

Sigma-Aldrich

氯化钙

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

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别名:
CaCl2, Calcium dichloride
线性分子式:
CaCl2
CAS号:
分子量:
110.98
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.71

等级

anhydrous

质量水平

蒸汽压

0.01 mmHg ( 20 °C)

产品线

BioReagent

检测方案

≥96.0%

形式

powder

technique(s)

cell culture | insect: suitable
cell culture | plant: suitable

mp

772 °C (lit.)

SMILES string

[Cl-].[Cl-].[Ca++]

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

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此商品
C49014497091.02379
Calcium chloride anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sigma-Aldrich

C5670

氯化钙

Essential+ Grade
Calcium chloride anhydrous, powder, ≥97%

Sigma-Aldrich

C4901

氯化钙

-
Calcium chloride AnhydroBeads™, −10 mesh, ≥99.9% trace metals basis

Sigma-Aldrich

449709

氯化钙

-
Calcium chloride anhydrous, granular ~ 1-2 mm

Supelco

1.02379

氯化钙

-
vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

-

assay

≥96.0%

assay

≥97%

assay

≥99.9% trace metals basis

assay

≥95.0% (complexometric)

form

powder

form

powder

form

-

form

solid

mp

772 °C (lit.)

mp

772 °C (lit.)

mp

772 °C (lit.)

mp

772 °C

product line

BioReagent

product line

-

product line

AnhydroBeads

product line

-

一般描述

氯化钙是一种常用的生化试剂。 钙在许多生物过程中起重要作用,如信号传导、肌肉收缩以及细胞膜和细胞壁的稳定性维持。1,2关于生物钙的实验测量已有大量文献发表。3,4氯化钙可用于感受态大肠杆菌的制备和转化,以及真核细胞的质粒DNA或高分子量基因组DNA转染。5已有研究显示质粒DNA pBR322可通过 CaCl2介导的电穿孔法导入大肠杆菌。6已有发表文献采用通过CaCl2浓缩病毒载体的实验方案。7CaCl2已用于大肠杆菌来源的NADH-泛醌氧化还原酶(复合体I)的稳定和二维结晶。8在CaCl2和柠檬酸钠存在下结晶的猪胰弹性蛋白酶显示其金属结合位点结合的是钙离子。9已通过多孔荧光法测定CaCl2对大肠杆菌微管蛋白样FtsZ分裂蛋白聚合化时总荧光强度的影响。10

应用

该产品已经过昆虫细胞培养测试(0.5 mg/L),证明其适用于昆虫细胞培养。氯化钙可用于感受态大肠杆菌的制备和转化,
以及真核细胞的质粒DNA或高分子量基因组DNA转染。已有研究显示质粒DNA pBR322可通过CaCl2介导的电穿孔法导入大肠杆菌。已有发表文献采用通过CaCl2浓缩病毒载体的实验方案。

制备说明

该产品可溶于水(222 mg/ml),形成澄清无色溶液。

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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钙在细胞培养中的应用

钙是一种离子稳定的二价阳离子,在细胞培养基中具有有益和有毒的双重属性,可作为一系列细胞培养基的组成部分。

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

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