C5867
Anti-Ciliated Cell Marker antibody, Mouse monoclonal
clone LhS 28, purified from hybridoma cell culture
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关于此项目
Conjugate:
unconjugated
Clone:
LhS 28, monoclonal
Application:
ARR, EM, ICC, IHC, WB
Citations:
2
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
LhS 28, monoclonal
form
buffered aqueous solution
mol wt
antigen ~45 kDa
species reactivity
human, hamster
concentration
~2 mg/mL
technique(s)
electron microscopy: suitable, immunocytochemistry: 1-2 μg/mL using BHK a21 cells, immunohistochemistry: suitable, microarray: suitable, western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
General description
Anti-Ciliated Cell Marker antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma LhS 28 produced by the fusion of mouse myeloma cells (Sp2/0 cells) and splenocytes from BALB/c mice.
Immunogen
BHK a21 cytoskeletal preparation.
Application
Anti-Ciliated Cell Marker antibody, Mouse monoclonal may be used in:
- immunoelectron microscopy
- immunoblotting
- immunohistochemistry
- immunocytochemistry
May be used in the detection of ciliated cells and for investigating mechanisms of ciliogenesis.
Biochem/physiol Actions
Anti-Ciliated Cell Marker antibody, Mouse monoclonal recognizes an antigen in human and hamster ciliated cells.
The mouse hybridoma LhS 28 secretes a monoclonal antibody that recognizes the antigen 44-45 kDa protein associated with the ciliary basal bodies. The antigen recognized by LhS 28 is present in the 9+0 microtubule arrays in the basal body structure of the cilium. It serves as an unequivocal marker for in situ detection and in vitro studies of human ciliated epithelial cells. LhS28 is a potential marker for tubal metaplasia identification and may be useful in recognizing low grade cervical glandular intraepithelial neoplasia. It is effective in detecting ciliogenesis and ciliated cells associated with normal and pathological human specimens
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
M T Comer et al.
Journal of clinical pathology, 52(5), 355-357 (1999-11-24)
The assessment of neoplastic disease in gynaecological histopathology can be complicated by the high incidence of metaplasia seen in tissues of the female genital tract. There is a need to identify specific tissue markers which can be applied in routine
M T Comer et al.
The Histochemical journal, 31(1), 39-43 (1999-07-16)
The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000-45,000 M(r) protein specifically associated
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