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Merck
CN

C6295

细胞冷冻培养基-DMSO 无血清 1x

sterile-filtered, suitable for cell culture

别名:

cryopreservation medium

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207
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产品名称

细胞冷冻培养基-DMSO 无血清 1x, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable
cryopreservation: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

Quality Level

Application

DMSO无血清1x细胞冷冻培养基已用于冷冻人类神经祖细胞(hNPCs),嗅鞘细胞(OECs)和视网膜色素上皮细胞(RPE)。

General description

细胞冷冻培养基是防冻剂DMSO与胎牛血清的组合。但无血清培养基可用甲基纤维素和泊洛沙姆188以及其他添加剂(如抗坏血酸、α-生育酚和脯氨酸)替代血清。

Other Notes

含 MEM、二甲基亚砜 (8.7%) 和甲基纤维素的完全即用型培养基。根据 Waymouth 的配方配制。

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse.
Waymouth, C. and Varnum, D.
TCA Manual, 2(1), 311-313 (1976)
Cryopreservation of cells using defined serum-free cryoprotective agents
Volbers JC, et al.
Current Directions in Biomedical Engineering, 2(1), 315-318 (2016)
Kai Pollard et al.
Scientific data, 7(1), 184-184 (2020-06-21)
Nerve sheath tumors occur as a heterogeneous group of neoplasms in patients with neurofibromatosis type 1 (NF1). The malignant form represents the most common cause of death in people with NF1, and even when benign, these tumors can result in
Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats.
Gowing G, et al.
Neuroreport, 25(6), 367-367 (2014)

商品

Overview of good cell banking practices for cell line cryopreservation purposes.

用于细胞系冷冻保存的良好细胞库操作实践概述。

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