无菌性
sterile-filtered
质量水平
表单
liquid
技术
cell culture | mammalian: suitable
cryopreservation: suitable
杂质
endotoxin, tested
运输
dry ice
储存温度
−20°C
一般描述
细胞冷冻培养基是防冻剂DMSO与胎牛血清的组合。但无血清培养基可用甲基纤维素和泊洛沙姆188以及其他添加剂（如抗坏血酸、α-生育酚和脯氨酸）替代血清。
应用
DMSO无血清1x细胞冷冻培养基已用于冷冻人类神经祖细胞(hNPCs)，嗅鞘细胞(OECs)和视网膜色素上皮细胞(RPE)。
其他说明
含 MEM、二甲基亚砜 (8.7%) 和甲基纤维素的完全即用型培养基。根据 Waymouth 的配方配制。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
J Yang et al.
Oncogene, 36(34), 4828-4842 (2017-04-11)
PIM1 is a proto-oncogene, encoding a serine/threonine protein kinase that regulates cell proliferation, survival, differentiation and apoptosis. Previous reports suggest that overexpression of PIM1 can induce cellular senescence. However, the molecular mechanism underlying this process is not fully understood. Here
Xiangwen Zhan et al.
Biochemical and biophysical research communications, 501(3), 779-785 (2018-05-16)
The proto-oncogene PIM1 encodes Ser/Thr kinase and regulates cell growth, differentiation and apoptosis. However, more and more studies including ours have found that PIM1 can induce senescence in normal human diploid fibroblasts and behave as a tumor suppressor. But the
Simple freezing procedure for storage in serum-free media of cultured and tumor cells of mouse.
Waymouth, C. and Varnum, D.
TCA Manual, 2(1), 311-313 (1976)
商品
用于细胞系冷冻保存的良好细胞库操作实践概述。
Overview of good cell banking practices for cell line cryopreservation purposes.
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