C6867
Anti-CENP-A antibody produced in rabbit
~2 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-CenH3, Anti-Centromere Protein A
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Conjugate:
unconjugated
Clone:
polyclonal
Application:
western blot
Species reactivity:
human
Citations:
7
Technique(s):
western blot: 1-2 μg/mL using HEK-293 cells expressing human CENP-A
Uniprot accession no.:
产品名称
Anti-CENP-A antibody produced in rabbit, ~2 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 17 kDa
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~2 mg/mL
technique(s)
western blot: 1-2 μg/mL using HEK-293 cells expressing human CENP-A
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CENPA(1058)
Application
Anti-CENP-A antibody has been used in immunoblotting.
Anti-CENP-A antibody produced in rabbit is suitable for western blotting at a concentration of 1-2μg/mL using HEK-293 cells expressing human CENP-A.
Biochem/physiol Actions
The location of the centromere on the chromosome is dependent on the histone H3 variant centromere protein A (CENP-A). The N-terminal tail of CENP-A is highly different from other H3 variants. Serine 7 of (CENP-A) plays an important role in mitosis-specific phosphorylation. It also plays a key role in kinetochore assembly. Alteration in CENP-A causes various human cancers. CENP-A may play an important role in epithelial ovarian cancer (EOC) and may serve as prognostic marker. It acts as a potential target for gene therapy in the treatment of EOC. The NH2 terminus of human CENP-A is essential for mitotic progression and necessary for the platform function of CENP-A centromeric chromatin in the assembly as well as maintenance of active kinetochores.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
CENP-A is a 17 kDa histone H3- like protein.
Centromere protein A is a protein encoded by the CENPA gene in humans. It is referred as CenH3 and CENP-A. It is a histone H3 variant that has centromere-specific nucleosomal property and is located at neocentromere found on a chromosome 10. CENP-A- binding clusters is associated with nucleosomal blocks within the neocentromeric chromatin.
Immunogen
synthetic peptide corresponding to amino acids 32-49 located near the N-terminus of human CENP-A, conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
常规特殊物品
此项目有
Degradation of nucleosome-associated centromeric histone H3-like protein CENP-A induced by herpes simplex virus type 1 protein ICP0
Lomonte P, et al.
Test, 276(8), 5829-5835 (2001)
R-M Liu et al.
Folia biologica, 59(3), 105-109 (2013-07-31)
Serine 7 of centromere protein A (CENP-A) is a very important mitosis-specific phosphorylation site. In this study, we demonstrate the subcellular distribution of Ser7 phosphorylated CENP-A during mitosis in MCF-7 cells. The Ser7 phosphorylation of CENP-A was observed beginning at
Jun-Jun Qiu et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 34(5), 2971-2975 (2013-05-29)
Altered expression of centromere protein-A (CENP-A) is observed in various types of human cancers. However, the clinical significance and pathological role of CENP-A in epithelial ovarian cancer (EOC) remains unclear. The main objective of this investigation was to clarify the
Hernan Diego Folco et al.
Science (New York, N.Y.), 319(5859), 94-97 (2008-01-05)
Heterochromatin is defined by distinct posttranslational modifications on histones, such as methylation of histone H3 at lysine 9 (H3K9), which allows heterochromatin protein 1 (HP1)-related chromodomain proteins to bind. Heterochromatin is frequently found near CENP-A chromatin, which is the key
Anderly C Chueh et al.
Human molecular genetics, 14(1), 85-93 (2004-11-13)
Human neocentromeres are fully functional centromeres that arise epigenetically from non-centromeric precursor sequences that are devoid of alpha-satellite DNA. Using chromatin immunoprecipitation (ChIP) and BAC-array analysis, we have previously described a 330 kb binding domain for CENP-A (a histone H3
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