C7482
Caspase 6 human
recombinant, expressed in E. coli, >90% (SDS-PAGE), lyophilized powder, 13,000 units/mg protein
别名:
Mch2
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Specific activity:
13,000 units/mg protein
Assay:
>90% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
assay
>90% (SDS-PAGE)
form
lyophilized powder
specific activity
13,000 units/mg protein
mol wt
~30 kDa
solubility
PBS: soluble
shipped in
dry ice
storage temp.
−70°C
Biochem/physiol Actions
Cleaves the substrates consisting of consensus sequence VEID.
Spontaneously undergoes autoprocessing to yield the subunits associated to form the active enzyme.
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates, or as a positive control in caspase activity assays.
Physical form
Contains 0.052% ammonium sulfate, 0.158% Tris−HCl, 0.76% sodium chloride.
Other Notes
One unit will hydrolyze 1 nmol of the caspase substrate VEID-pNA to VEID and p-nitroaniline per hour at pH 7.2 at 37 °C.