产品名称
伴侣蛋白60 来源于大肠杆菌, >95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder
biological source
Escherichia coli
recombinant
expressed in E. coli overproducing strain
assay
>95% (SDS-PAGE)
form
lyophilized powder
technique(s)
electron microscopy: suitable
mass spectrometry (MS): suitable
suitability
passes test (Refolding (w/GroES))
UniProt accession no.
storage temp.
2-8°C
Quality Level
Gene Information
human ... HSPD1(3329)
Application
来自大肠埃希菌的伴侣蛋白60已用于:
- 质谱
- 作为测试颗粒分布的对照品用于冷冻电子显微镜成像
- 作为红外光谱测量的标准品
- 以差分迁移率分析(DMA)和冷冻电子显微镜(cryo-EM)进行的适用性评估和表征研究中的蛋白质样品。
Biochem/physiol Actions
除了促进蛋白质折叠的作用外,伴侣蛋白60(Cpn60)可作为胞外信号传导蛋白,其功能类似于某些促炎性细胞因子。此外,Cpn60有助于抑制细胞分化初期的脂质蓄积和脂肪生成,从而具有抗肥胖作用。
General description
研究领域:细胞信号传导
伴侣蛋白60(GroEL)和伴侣蛋白10(GroES)属于在原核生物和真核细胞器中普遍存在的热休克分子伴侣家族。
伴侣蛋白60(GroEL)和伴侣蛋白10(GroES)属于在原核生物和真核细胞器中普遍存在的热休克分子伴侣家族。
Packaging
包装尺寸基于蛋白质含量。
Physical form
含有Tris缓冲盐、氯化钾、氯化镁、二硫苏糖醇和海藻糖作为稳定剂的冻干粉末。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Infrared irradiation in the collision cell of a hybrid tandem quadrupole/time-of-flight mass spectrometer for declustering and cleaning of nanoelectrosprayed protein complex ions
El-Faramawy A, et al.
Analytical Chemistry, 82(23), 9878-9884 (2010)
Observation of a single protein by ultrafast X-ray diffraction
Ekeberg T, et al.
Light: Science & Applications, 13(1), 15-15 (2024)
Chaperonin 60 unfolds its secrets of cellular communication
Maguire M, et al.
Cell Stress & Chaperones, 7(4), 317-329 (2002)
Separating and visualising protein assemblies by means of preparative mass spectrometry and microscopy
Benesch JLP, et al.
Journal of Structural Biology, 172(2), 161-168 (2010)
Surface-exposed chaperonin 60 derived from Propionibacterium freudenreichii MJ2 inhibits adipogenesis by decreasing the expression of C/EBP?/PPAR?
An M and Lim Y-H
Scientific Reports, 13(1), 19251-19251 (2023)
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