C7867
Anti-claspin (C-terminal) 兔抗
IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 250 kDa (170 kDa band that may correspond to a claspin related protein is observed in some cell lines)
species reactivity
human
technique(s)
microarray: suitable, western blot: 1:250-1:500 using K562 cell extracts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CLSPN(63967)
Immunogen
synthetic peptide corresponding to amino acids 1321-1339 of human claspin, conjugated to KLH via an N-terminal added cysteine residue.
Application
Anti-Claspin (C-terminal) antibody is suitable for microarray and western blot analysis at a dilution of 1:250-1:500.
Biochem/physiol Actions
Claspin is a protein which plays a major role in DNA replication. It involves in protecting DNA replication forks against DNA damage. In DNA damage response, claspin directly interacts with Rad17, which is required for ATR activation. ATR is activated by various DNA damage and DNA replication stress. Once activated, ATR (ataxia telangiectasia mutated-Rad3 related protein) phosphorylates effector kinase Chk1 which further depends on mediator protein claspin. Claspin helps to stimulate phosphorylation reaction of Chk1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
A Kumagai et al.
Molecular cell, 6(4), 839-849 (2000-11-25)
We have identified Claspin, a novel protein that binds to Xenopus Chk1 (Xchk1). Binding of Claspin to Xchk1 is highly elevated in the presence of DNA templates that trigger a checkpoint arrest of the cell cycle in Xenopus egg extracts.
Shizhou Liu et al.
Molecular and cellular biology, 26(16), 6056-6064 (2006-08-02)
TopBP1 and Claspin are adaptor proteins that facilitate phosphorylation of Chk1 by the ATR kinase in response to genotoxic stress. Despite their established requirement for Chk1 activation, the exact way in which TopBP1 and Claspin control Chk1 phosphorylation remains unclear.
Shizhou Liu et al.
Cell cycle (Georgetown, Tex.), 11(14), 2711-2716 (2012-06-27)
Claspin is a key mediator of the ATR-Chk1 checkpoint pathway. In response to DNA damage, Claspin interacts with Rad17 and Chk1 in a phosphorylation-dependent manner, enabling ATR to phosphorylate Chk1 efficiently. Claspin also interacts with Rad9, but how they interact
Yasunori Fukumoto et al.
The Journal of biological chemistry, 289(18), 12313-12329 (2014-03-19)
The DNA damage checkpoint arrests cell cycle progression to allow time for repair. Once DNA repair is completed, checkpoint signaling is terminated. Currently little is known about the mechanism by which checkpoint signaling is terminated, and the disappearance of DNA
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