表单
buffered aqueous glycerol solution
比活
50,000-150,000 units/mg protein
分子量
75 kDa (three identical subunits)
运输
wet ice
储存温度
−20°C
基因信息
Escherichia coli ... cat(2847485)
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应用
Chloramphenicol acetyltransferase from Escherichia coli has been used in a study to assess the construction of a novel expression system in Klebsiella pneumoniae and its application for 1,3-propanediol production. Chloramphenicol acetyltransferase from Escherichia coli has also been used in a study to investigate site-directed mutagenesis and promoter functional analysis of the RM07 DNA fragment from Halobacterium halobium.
The enzyme has been used in chloramphenicol acetyltransferase assay to optimize the transfection of plasmid DNA into primary cultures of adult mouse keratinocytes. It has also been used to assess the acetyl-CoA carboxylase-carboxyltransferase (ACC-CT) domain activity. This has been done using a coupled-two phase system measuring the selective partition of [14C]acetylchloramphenicol into an organic layer.
生化/生理作用
这种酶是细菌对氯霉素产生抗性的原因。催化乙酰辅酶 A 和氯霉素转化为辅酶 A 和氯霉素 3-乙酸酯。
单位定义
在 pH 7.8 和 25°C 下，一单位本品每分钟可以将 1.0 纳摩尔氯霉素和乙酰辅酶 A 转化为氯霉素 3-乙酸酯和辅酶 A。
外形
50% 甘油中的透明无色溶液，含 5mM Tris-HCl (pH 7.8) 和 0.5mM 2-巯基乙醇
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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