C8890
4-氯-1-萘酚
horseradish peroxidase substrate, fluorogenic, ≥98% (GC), crystalline
别名:
4-chloronaphthalen-1-ol
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关于此项目
线性分子式:
ClC10H6OH
化学文摘社编号:
分子量:
178.61
Beilstein:
509750
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83
产品名称
4-氯-1-萘酚, crystalline
方案
≥98% (GC)
质量水平
表单
crystalline
mp
118-121 °C (lit.)
溶解性
acetone: 50 mg/mL, clear, colorless to very faintly brown
储存温度
−20°C
SMILES字符串
Oc1ccc(Cl)c2ccccc12
InChI
1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
InChI key
LVSPDZAGCBEQAV-UHFFFAOYSA-N
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一般描述
Chromogenic peroxidase substrate useful in enzyme-linked detection procedures.
应用
4-Chloro-1-naphthol has been used to visualize protein bands in western blotting. It has also been used in staining polyvinylidene fluoride (PVDF) membrane by Nakane method.
生化/生理作用
4-Chloro-1-naphthol serves as a substrate for horseradish peroxidase.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Gloves
Limited proteolysis of fibrinogen by fibrinogenase from Echis multisquamatis venom
Chernyshenko VO
The Protein Journal, 34(2), 130-134 (2015)
Endothelial microparticles induce formation of platelet aggregates via a von Willebrand factor/ristocetin dependent pathway, rendering them resistant to dissociation
JIMENEZ JJ, et al.
Journal of Thrombosis and Haemostasis, 3(6), 1301-1308 (2005)
Visualization under ultraviolet light enhances 100-fold the sensitivity of peroxidase-stained blots.
A Domingo et al.
Analytical biochemistry, 182(1), 176-181 (1989-10-01)
As described in this article, visualization and/or photography under uv light of 4-chloro-1-naphthol-developed, peroxidase-marked immunoblots allows an increase in sensitivity of more than 100 times over the apparent staining results observable under normal visible white light. This increase in sensitivity
D J Litman et al.
Clinical chemistry, 29(9), 1598-1603 (1983-09-01)
We describe an internally referenced immunochemical test-strip for use in the rapid detection of morphine. The method is based on the "enzyme-channelling" immunoassay technique, and a glucose oxidase-horseradish peroxidase enzyme pair is used to immunospecifically generate an insoluble, colored reaction
R Kobayashi et al.
Analytical biochemistry, 183(1), 9-12 (1989-11-15)
A method which allows the highly sensitive and simple immunodetection of antigen-antibody complexes on nitrocellulose papers has been developed. The method is a modification of the procedure known as the "Nadi reaction" (oxidative coupling of 1-naphthol and N,N'-dimethyl-p-phenylendiamine) for histochemical
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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