生物来源
rat tail
表单
powder
技术
cell culture | mammalian: suitable
溶解性
aqueous acid: soluble
UniProt登记号
储存温度
2-8°C
基因信息
rat ... Col1a1(29393)
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应用
该产品旨在在组织培养板上产生薄层涂层，以促进贴壁依赖性细胞的附着，推荐用量为 6-10 μg/cm2。 本品不能用于制作3-D凝胶。 I型胶原常作为细胞培养的附着基质，与成肌细胞、脊髓神经节、肝细胞、胚胎肺、心脏外植体、成纤维细胞、内皮细胞、胰岛细胞一起在I型胶原的膜或凝胶上成功培养。I型胶原也可用于特发性肺纤维化(IPF)的研究，研究内质网应激IPF对肺成纤维细胞的影响。 酸性溶液中的胶原蛋白可以产生三维支架，用于生物工程和细胞培养。
生化/生理作用
I型胶原蛋白是皮肤、骨骼、肌腱和其他纤维结缔组织的组成部分。
制备说明
本品是通过改良的Bornstein提取方法(Bornstein, M.B., Lab. Invest., 7, 134 (1958))而制备的。 作为酸溶性胶原, 可溶于pH 3.0的乙酸水溶液中(5 mg/mL乙酸调节），生成乳白色无色溶液。
其他说明
所有胶原蛋白分子均由以三螺旋构象排列的三条肽链组成，其一级结构主要以三个氨基酸作为重复基序，即每隔两个氨基酸是一个甘氨酸残基，其前面的两个位置通常为脯氨酸或4-羟基脯氨酸。 I型胶原不同于其他胶原蛋白的方面为其低赖氨酸羟基化和低碳水化合物构成。
胶原蛋白被分为许多种结构和遗传性不同的类型。我们使用Bornstein和Traub提出的命名法。不要混淆Sigma分类标识和公认的胶原蛋白分类类型。
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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