产品名称
伴刀豆球蛋白A 来源于洋刀豆 （刀豆）, Sepharose™ conjugate, buffered aqueous suspension
Quality Level
biological source
Canavalia ensiformis
conjugate
Sepharose™ conjugate
form
buffered aqueous suspension
contains
20% ethanol as preservative
extent of labeling
10-16 mg per mL
matrix
Sepharose 4B
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥25 mg/mL binding capacity (thyroglobulin)
storage temp.
2-8°C
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Analysis Note
在报道中，凝集活性以μg/ml表示，并且是在含有1mg/mL Ca 2+和Mn 2+的磷酸盐缓冲盐水(pH 6.8)的系列稀释液中进行测定的。该活性是在25℃孵育1小时后，对2％人红细胞悬浮液进行凝集所需的最低浓度。
Application
用于纯化含甘露糖的糖蛋白。
Biochem/physiol Actions
刀豆素 A 无血型特异性，但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时，刀豆素 A 以四聚体形式存在；pH 高于 7.0 时，形成更高的聚合体。根据聚合程度不同，刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。
Physical form
悬浮于0.1 M醋酸盐缓冲液中，pH 6.0，含有1 M NaCl以及 CaCl2、MgCl2和MnCl2各1 mM
Legal Information
Sepharose is a trademark of Cytiva
signalword
Danger
hcodes
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
存储类别
3 - Flammable liquids
wgk
WGK 3
flash_point_f
102.2 °F
flash_point_c
39 °C
法规信息
动植物源性产品
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