form
lyophilized powder
Quality Level
technique(s)
affinity chromatography: suitable
matrix
cross-linked 4% beaded agarose
matrix active group
cyanate or related structures
matrix spacer
1 atom (when ligands are coupled via isourea derivative or related linkage)
swelling
1 g swells to 2.5-4.5 mL
capacity
≥10 mg/mL binding capacity (BSA)(via amino groups)
suitability
suitable for chromatography
storage temp.
−20°C
Application
氰化溴化物活化的琼脂糖是一种用乳糖稳定的冻干粉,可用于亲和层析、蛋白层析、蛋白相互作用、抗体标记、抗体修饰以及将抗体附着于琼脂糖珠上。
Physical form
用乳糖稳定化的冻干粉
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
N A Morjana et al.
Protein expression and purification, 5(2), 144-148 (1994-04-01)
Protein disulfide isomerase (PDI) catalyzes the formation and rearrangement of disulfide bonds during protein folding. PDI coupled to cyanogen bromide-activated agarose retains its catalytic activity, and a column of this material increases both the rate and the yield for folding
Immobilization of solubilized UDP-glucuronosyltransferase from rat liver microsomes to Sepharose 4B.
R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of
C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support.
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| C9210-15G | 04061833523278 |
| C9210-5G | 04061833523582 |
| C9210-1G | 04061833523285 |
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