C9697
胶原酶 来源于溶组织梭菌
lyophilized powder (from 0.2 μm filtered solution), suitable for cell culture
别名:
梭菌肽酶 A
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
Specific activity:
≥800 units/mg solid
Biological source:
Clostridium histolyticum
Concentration:
1 mg/mL
biological source
Clostridium histolyticum
sterility
0.2 μm filtered
form
lyophilized powder (from 0.2 μm filtered solution)
specific activity
≥800 units/mg solid
mol wt
68-130 kDa
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
pH
7.4
storage temp.
−20°C
Quality Level
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General description
胶原酶是一种蛋白酶，可切割被称为胶原蛋白的三螺旋蛋白。组织胶原酶有三种类型，它们属于基质金属蛋白酶（MMP）家族。来源于溶组织梭菌（Clostridium histolyticum）的胶原酶具有非常强的活性，可在低至4-10℃的温度下从两端消化胶原蛋白。粗制胶原酶混合物主要含有两种的酶，即胶原酶和梭菌蛋白酶。
Application
来自溶组织梭菌（ Clostridium histolyticum ）的胶原酶被用于以下应用：
本品适用于人类肿瘤、小鼠肾脏、人类成人和胎儿的脑、肺和许多其他上皮组织的解离。它还被证明适用于肝脏和肾脏灌注研究、胰腺消化以及肝细胞制备。胶原酶也已用于制备动脉组织，以对晚期糖基化终产物进行研究。该酶用于（浓度约为1mg/ml）对肝细胞的释放进行检测。消化浓度范围为0.1至5mg/mL。
- 足细胞分离
- 用于酶催化法的对比
- 用于免疫细胞化学的组织制备
- 睾丸精子提取
- 制备单细胞悬浮液
- 免疫荧光法
本品适用于人类肿瘤、小鼠肾脏、人类成人和胎儿的脑、肺和许多其他上皮组织的解离。它还被证明适用于肝脏和肾脏灌注研究、胰腺消化以及肝细胞制备。胶原酶也已用于制备动脉组织，以对晚期糖基化终产物进行研究。该酶用于（浓度约为1mg/ml）对肝细胞的释放进行检测。消化浓度范围为0.1至5mg/mL。
Biochem/physiol Actions
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
胶原酶产品是溶组织梭菌（Clostridium histolyticum）分泌的酶混合物，不同的产品可通过分泌酶中10-18种组分的相对比例进行区分。主要成分是两种胶原酶，即梭菌蛋白酶和中性蛋白酶。这些酶的协同作用可降解胶原蛋白和其他细胞内物质。胶原酶和中性蛋白酶的作用对于从组织中有效释放细胞来说都是必需的。各种类型的胶原蛋白均是胶原酶的天然底物。
Preparation Note
将XI型胶原酶以1-2 mg/mL溶于TESCA缓冲液（含有50mM TES，0.36mM氯化钙，pH 7.4，37℃）制备胶原酶溶液。
Other Notes
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
Disclaimer
未开封的产品可在-20°C下稳定保存一年。在37℃、50℃和-20℃下储存30天，FALGPA或蛋白酶活性无损失。如果将粗制胶原酶溶液分装（10 mg/mL）后快速冷冻并冷藏在-20℃下，可稳定保存。后续的冻融循环将破坏溶液。若将本品放在冰上，可在7小时内保持100%活性。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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商品
Discover pre-mixed collagenase enzyme blends with DNase I, Dispase II, Elastase, and Hyaluronidase and gently dissociate animal tissues in vitro.
实验方案
To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.
在测定胶原酶活性时，使用N-（3-[2-呋喃基]丙烯酰基）-Leu-Gly-Pro-Ala在345nm处进行连续分光光度法测定。胶原酶水解胶原肽键。
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