Name: Cholera Toxin B subunit
Brand: SIGMA

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About This Item

MDL编号:

MFCD02356522

UNSPSC代码:

12352200

PubChem化学物质编号:

329775212

NACRES:

NA.77

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Product Name

Cholera Toxin B subunit, ≥95% (SDS-PAGE), lyophilized powder

质量水平

300

方案

≥95% (SDS-PAGE)

表单

lyophilized powder

分子量

~12 kDa

组成

Protein, ~5% Lowry

杂质

≤0.5% Cholera toxin A subunit (SDS-PAGE)

溶解性

H₂O: 10 mg/mL

储存温度

2-8°C

SMILES字符串

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

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一般描述

Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.

应用

Cholera Toxin B subunit has been used:

for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

生化/生理作用

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

特点和优势

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

包装

Package size based on protein content

外形

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.

制备说明

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN₃ and 1 mM sodium EDTA.

分析说明

Activity measured by ELISA using ganglioside GM₁-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.