生物来源
dog kidney
包装
tube of 5 μg 02050101-DNA-5UG
pkg of vial of cells 02050101-1VL
生长模式
Aggregates in suspension
核型
Not specified
形态学
Not specified
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系来源
Canine Cocker Spaniel kidney
细胞系描述
MDCK cell line (Sigma Catalogue number. 84121903) adapted to grow in the protein-free medium ′MDCK Protein-free Medium′ (Sigma M3678). Stirred culture is recommended, cells grow in suspended clumps.
应用
Propagation of viruses in the absence of animal-derived proteins.
培养基
MDCK Protein-free medium (Sigma M3678) + 3.3mM Glutamine. When freezing cells down use Cell Freezing Medium (Sigma C2639) + 10% DMSO or use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
传代培养常规
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
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