biological source
dog kidney
packaging
tube of 5 μg 02050101-DNA-5UG, pkg of vial of cells 02050101-1VL
growth mode
Aggregates in suspension
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Propagation of viruses in the absence of animal-derived proteins.
Biochem/physiol Actions
Canine Cocker Spaniel kidney
MDCK cell line (Sigma Catalogue number. 84121903) adapted to grow in the protein-free medium ′MDCK Protein-free Medium′ (Sigma M3678). Stirred culture is recommended, cells grow in suspended clumps.
Preparation Note
MDCK Protein-free medium (Sigma M3678) + 3.3mM Glutamine. When freezing cells down use Cell Freezing Medium (Sigma C2639) + 10% DMSO or use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
Other Notes
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法规信息
低风险生物材料
动植物源性产品
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