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Merck
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About This Item

UNSPSC代码:
41106514
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生物来源

dog kidney

包装

tube of 5 μg 02050101-DNA-5UG
pkg of vial of cells 02050101-1VL

生长模式

Aggregates in suspension

核型

Not specified

形态学

Not specified

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

细胞系来源

Canine Cocker Spaniel kidney

细胞系描述

MDCK cell line (Sigma Catalogue number. 84121903) adapted to grow in the protein-free medium ′MDCK Protein-free Medium′ (Sigma M3678). Stirred culture is recommended, cells grow in suspended clumps.

应用

Propagation of viruses in the absence of animal-derived proteins.

培养基

MDCK Protein-free medium (Sigma M3678) + 3.3mM Glutamine. When freezing cells down use Cell Freezing Medium (Sigma C2639) + 10% DMSO or use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

传代培养常规

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

其他说明

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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