biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 03062503-DNA-5UG, pkg of vial of cells 03062503-1VL
ImmunoDon
BALB/c mouse
Myeloma
NS1
Reactivity
Anti-CD47, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Biotinylated rat (AO) thymocytes
Biochem/physiol Actions
Mouse NS1 x mouse BALB/c
This antibody recognises the widely distributed CD47 (integrin associated protein), the ligand of SIRPα.
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Seed at 5 x 10,000 cells/ml. Subculture when cells reach 500,000 - 1,000,000 cells/ml (usually after 3-4 days) 5% CO2 37° C. Start in 20% FBS when recovering from frozen.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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