biological source
Mouse x Mouse Hybridoma
packaging
tube of 5 μg 03062503-DNA-5UG, pkg of vial of cells 03062503-1VL
ImmunoDon
BALB/c mouse
Myeloma
NS1
Reactivity
Anti-CD47, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Biotinylated rat (AO) thymocytes
Biochem/physiol Actions
Mouse NS1 x mouse BALB/c
This antibody recognises the widely distributed CD47 (integrin associated protein), the ligand of SIRPα.
Preparation Note
Seed at 5 x 10,000 cells/ml. Subculture when cells reach 500,000 - 1,000,000 cells/ml (usually after 3-4 days) 5% CO2 37° C. Start in 20% FBS when recovering from frozen.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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