biological source
rat nose
packaging
tube of 5 μg 03071601-DNA-5UG, pkg of vial of cells 03071601-1VL
growth mode
Adherent
karyotype
Normal rat chromosome complement
morphology
Epithelial-like
products
cadherin, tenascin, laminin, N-CAM, GFAP, p75 NTR
receptors
p75 NTR
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Neural research including axonal regeneration.
Biochem/physiol Actions
Adult rat olfactory nerve ensheathing cells
Rolf B1.T cells arose from a spontaneous immortalisation event - retaining characteristics of normal cells. The cells are dependent on serum growth factors for growth. They constitutively express glial fibrillary acidic protein, S100, the low affinity neurotrophin receptor p75 NTR, laminin, tenascin, and NCAM. These cells support the regrowth of neurites from adult rat retinal ganglion cells. They have potential value in research into mechanisms of glial support for axonal regeneration from adult mammalian central neurons and other aspects of differentiated glial cell biology. Epithelial-like or fusiform (inducible).
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:5 - 1:20 e.g. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.