Sp2/0-Ag14-TurboDoma

Fusion partner for generation of serum-free hybridomas, host for the expression of recombinant genes.

Mouse x Mouse hybridoma, non-producing, serum-free

Sp2/0-Ag14 cell line (Sigma Catalogue number. 85072401) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma (Cell Culture Technologies). Suitable for use as a fusion parent for serum-free generation of hybridomas as well as a host for the production of recombinant proteins.

TurboDoma^™ medium + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C..

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TurboDoma is a trademark of Cell Culture Technologies