biological source
African green monkey kidney
packaging
tube of 5 μg 03092503-DNA-5UG, pkg of vial of cells 03092503-1VL
growth mode
Adherent
karyotype
Not Specified
morphology
Fibroblast-like
receptors
Not specified
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Biochem/physiol Actions
African green monkey kidney cells, serum-free
Vero cell line (Sigma catalog no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent and may be suitable for the replication of viral particles.
Preparation Note
Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513). When freezing cells down use Hektor G medium + 4 mM L-Glutamine (G7513) + 10% DMSO (D2650).
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2. Use EDTA only (no trypsin); 5% CO2; 37 °C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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