生物来源
insect ovary
包装
tube of 5 μg 05022801-DNA-5UG
pkg of vial of cells 05022801-1VL
生长模式
Adherent
核型
Not specified
形态学
Spherical
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Protein expression using the Baculovirus vector system
生化/生理作用
Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.
Spodoptera frugiperda pupal ovarian tissue
制备说明
SF90011 + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm2; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.
其他说明
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法规信息
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Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.
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