biological source
insect ovary
packaging
tube of 5 μg 05022801-DNA-5UG, pkg of vial of cells 05022801-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Spherical
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Protein expression using the Baculovirus vector system
Biochem/physiol Actions
Derived from pupal ovarian tissue of spodoptera frugiperda. The cells are highly susceptible to Baculovirus infection and are used in the production of protein products genetically manipulated into Baculovirus vector systems. They were derived at the USDA Insect Pathology Laboratory from a cell line originating from pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. Sf21 cells have greater range in size and have a doubling time of 24 hr compared to 48-72 hr for Sf9 cells.
Spodoptera frugiperda pupal ovarian tissue
Preparation Note
SF90011 + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x10,000 cells/cm2; 28 C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, centrifuge suspension and resuspend cell pellet in fresh medium.
Other Notes
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Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.
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