biological source
hamster kidney
packaging
tube of 5 μg 05062301-DNA-5UG, pkg of vial of cells 05062301-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
BHK21CL13-2P cell line (Sigma Catalogue number. 84111301) adapted to grow in the chemically defined protein- and peptide-free Hektor™ G medium.
Hamster Syrian kidney, serum-free
Preparation Note
Hektor™ G medium + 4mM Glutamine + 5 ug/ml Insulin. When freezing cells down use Hektor G medium + 4mM Glutamine (50:50 conditioned medium:fresh medium) + 10% DMSO.
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Legal Information
Hektor is a trademark of Cell Culture Technologies
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