biological source
human forehead teratoma
manufacturer/tradename
ECACC
packaging
tube of 5 μg 06011802-DNA-5UG, pkg of vial of cells 06011802-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Teratoma
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
An undifferentiated carcinoma cell line established from a forehead metastasis of a malignant teratoma. This is not an embryonal carcinoma cell line. This cell line is tumourigenic in nude mice.
Human forehead metastasis of malignant teratoma
Preparation Note
DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin or trypsin/EDTA; 10% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.