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UNSPSC Code:
41106514
Biological source:
human prostate (metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
Refer to reference (Korenchuk et al., 2001)
Morphology:
Epithelial
biological source
human prostate (metastasis)
packaging
tube of 5 μg 06020201-DNA-5UG, pkg of vial of cells 06020201-1VL
growth mode
Adherent
karyotype
Refer to reference (Korenchuk et al., 2001)
morphology
Epithelial
products
Not specified
receptors
Androgen receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human Prostate Cancer Metastasis
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19
This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.
Preparation Note
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.
Other Notes
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