LA1-55n

Derived from LA1-5s a clonal subline of LA-N-1. LA-N-1 was derived from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma.LA-N-1 (Sigma Catalogue number 06041201), LA1-55n (Sigma Catalogue number 06041203) and LA1-5s (Sigma Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Human Neuroblastoma

EMEM (with non-essential amino acids) and Ham′s F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 to 1:100 ratio; 8% CO₂, 37°C. Cells are moderate to weakly adherent. Allow floating clumps to settle then withdraw medium. Remove at

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