biological source
human colon
packaging
tube of 5 μg 06061902-DNA-5UG, pkg of vial of cells 06061902-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from a colon adenocarcinoma of a 58 year-old female patient. The adenocarcinoma was moderately differentiated and determined to be Dukes′ stage B. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.
Human colon adenocarcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 9,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16
CSF1PO: 11,14
D13S317: 9
D16S539: 10,11
D5S818: 9,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,10
vWA: 14,16
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:2 to 1:10 using 0.05% trypsin/EDTA; 7.5% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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