biological source
human eye
packaging
tube of 5 μg 06070601-DNA-5UG, pkg of vial of cells 06070601-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Clusters of round cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human Retinoblastoma
Human retinoblastoma cell line derived from a male patient. Bilateral retinoblastoma, tumour treated by irradiation. Tumourigenicity not tested.
Preparation Note
Iscove′s Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO2; 37°C. Saturation density: 2 x 1000,000 cells/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.