biological source
human eye
packaging
tube of 5 μg 06070602-DNA-5UG, pkg of vial of cells 06070602-1VL
growth mode
Semi-adherent
karyotype
Not specified
morphology
Grape like clusters of round cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human Retinoblastoma
Human retinoblastoma cell line derived from a female Caucasian. The cells are semi-adherent, some neuronal differentiation can occur when subculturing. Tumourigenic in rabbits.
Preparation Note
Iscove′s Modified DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS) + 10ug/ml Insulin + 55uM 2-Mercaptoethanol
Split subconfluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-3 x100,000 cells/ml; 5% CO2; 37°C. Saturation density: 2 x 1000,000 cells/ml. Cells are semi-adherent. Scrape cells from surface; do not use trypsin.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.