biological source
human embryo (carcinoma)
packaging
tube of 5 μg 06072609-DNA-5UG, pkg of vial of cells 06072609-1VL
growth mode
Not specified
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
-140 to -196°C
Biochem/physiol Actions
A human embryonal carcinoma cell line established from a primary testicular tumour of a 22 year-old patient. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection.
Human embryonal carcinoma
STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 11,14
D5S818: 11,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,17
Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 11,14
D5S818: 11,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,17
Preparation Note
DMEM (High Glucose) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). No sodium pyruvate.
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. Cells need to be maintained at a high cell density.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.