biological source
human embryo (carcinoma)
packaging
tube of 5 μg 06072609-DNA-5UG, pkg of vial of cells 06072609-1VL
growth mode
Not specified
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Biochem/physiol Actions
A human embryonal carcinoma cell line established from a primary testicular tumour of a 22 year-old patient. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection.
Human embryonal carcinoma
STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 11,14
D5S818: 11,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,17
Amelogenin: X
CSF1PO: 11
D13S317: 8,9
D16S539: 11,14
D5S818: 11,12
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16,17
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 10% CO2; 37°C. Cells need to be maintained at a high cell density.
DMEM (High Glucose) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS). No sodium pyruvate.
Other Notes
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