biological source
human blood (leukemia)
packaging
tube of 5 μg 06120601-DNA-5UG, pkg of vial of cells 06120601-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Single round to polymorphic cells growing in suspension
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
-140 to -196°C
Biochem/physiol Actions
Established from peripheral blood of a 71 year-old woman at diagnosis of B-cell acute lymphoblastic leukemia (ALL). No rearrangement of C-MYC DNA sequences.
Human B-cell leukemia
Not specified
Preparation Note
Maintain cultures between 0.5 - 2 x1000,000 cells/ml; 5% CO2; 37 °C. Doubling time approximately 60 hours. Split saturated culture 1:2 every 2-4 days. Cells are single round to polymorphic and grow in suspension.
RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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