biological source
mouse esophagus
description
Mouse SIM strain embryonic fibroblast
packaging
tube of 5 μg 07032801-DNA-5UG, pkg of vial of cells 07032801-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Mouse SIM strain embryonic fibroblast
SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.
Preparation Note
Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;5% CO2; 37°C.
Other Notes
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法规信息
动植物源性产品
低风险生物材料
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