产品名称
Vero (AC-free), NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
African green monkey kidney
packaging
tube of 5 μg 08011101-DNA-5UG
pkg of vial of cells 08011101-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
receptors
Not specified
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Biochem/physiol Actions
African green monkey kidney cells, serum-free
Vero cell line (Sigma catalog no. 84113001) adapted to grow in animal component free medium (VP-SFM; Gibco catalog no. 11681-020).
General description
Vero cells are anchorage-dependent cells derived from the kidney of African green monkey cells. They have applications in molecular and cell biology research. Vero cells are a useful host for the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Preparation Note
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.
VP-SFM (Gibco cat no. 11681-020) + 4 mM L-Glutamine (G7513). When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).
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