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关于此项目
UNSPSC Code:
41106514
Biological source:
human brain
Relevant disease(s):
cancer
Growth mode:
Not specified
Karyotype:
Near tetraploid; range 88 to 91 - see Cell Line Description for more information
Morphology:
Fusiform, can form heaps of cells
biological source
human brain
packaging
tube of 5 μg 08040401-DNA-5UG, pkg of vial of cells 08040401-1VL
growth mode
Not specified
karyotype
Near tetraploid; range 88 to 91 - see Cell Line Description for more information
morphology
Fusiform, can form heaps of cells
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)
Human glioblastoma
Preparation Note
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37ºC.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.