N18 (Hamprecht)
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
生物来源
mouse nerve
包装
tube of 5 μg 08062527-DNA-5UG
pkg of vial of cells 08062527-1VL
生长模式
Adherent
核型
Not specified
形态学
Not specified
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Can be used as a neuronal model
生化/生理作用
Mouse neuroblastoma
N18 is a clonal line produced from the mouse neuroblastoma C1300. (Mouse strain A/Jax). ECACC has two catalogue entries for the N18 cell line which differ in that they were deposited by different groups: N18 from the original group that derived the cell line (Sigma Catalogue number 88112301) and N18(Hamprecht) (Sigma Catalogue number. 08062527). Several other cell lines in the ECACC catalogue have been derived from N18(Hamprecht).
制备说明
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
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