N18 (Hamprecht)

Can be used as a neuronal model

Mouse neuroblastoma

N18 is a clonal line produced from the mouse neuroblastoma C1300. (Mouse strain A/Jax). ECACC has two catalogue entries for the N18 cell line which differ in that they were deposited by different groups: N18 from the original group that derived the cell line (Sigma Catalogue number 88112301) and N18(Hamprecht) (Sigma Catalogue number. 08062527). Several other cell lines in the ECACC catalogue have been derived from N18(Hamprecht).

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS)

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.