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UNSPSC Code:
41106514
Biological source:
rat brain
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
modal No varied with passage see reference
Morphology:
fibroblastic
biological source
rat brain
packaging
tube of 5 μg 08062528-DNA-5UG, pkg of vial of cells 08062528-1VL
growth mode
Adherent
karyotype
modal No varied with passage see reference
morphology
fibroblastic
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
electrophysiology
Biochem/physiol Actions
A pseudotetraploid call line generated by fusion of 8-bromodeoxyuridine-resistant mutant rat glioma cell line C6-BU-1 and the 6-thioguanine-resistant mutant rat glioma cell line C6-TG-1.
Rat glioma x rat glioma hybrid
Preparation Note
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C
Other Notes
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