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UNSPSC Code:
41106514
Biological source:
mouse nerve (fibroblast)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Modal No. varied with passage see reference
Morphology:
Neuronal/fibroblastic
biological source
mouse nerve (fibroblast)
packaging
tube of 5 μg 08062531-DNA-5UG, pkg of vial of cells 08062531-1VL
growth mode
Adherent
karyotype
Modal No. varied with passage see reference
morphology
Neuronal/fibroblastic
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Used as a neuronal Model
Biochem/physiol Actions
Hybrid generated by fusion of 6-thioguanine-resistant mutant of mouse neuroblastoma N4TG3 (Sigma Catalogue number 08062515) and 8-bromodeoxyuridine-resistant mutant of mouse L cell fibroblast line B82 (Sigma Catalogue number 08062522) using inactivated Sendai virus. The fusion of N4TG3 x B82 generated two types of hybrid cells: neuronal resembling the neuroblastoma and epithelioid with clearly visible nucleus. 328/8 is of the neuronal type.
Mouse neuroblastoma x mouse L cell fibroblast hybrid
Preparation Note
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days
Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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