108CC5-TG-3

Used as a neuronal Model

A 6-thioguanine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid

Mouse neuroblastoma x Rat glioma hybrid

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine. The cells can also be grown in the absence of 6-thioguanine.

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required.

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