biological source
human lung
packaging
tube of 5 μg 09100101-DNA-5UG, pkg of vial of cells 09100101-1VL
growth mode
Adherent
karyotype
Number of chromosomes 72-75
morphology
Spindle shaped cells
products
Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
Biochem/physiol Actions
A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18
Preparation Note
Hams F10 + 15% FCS + 2mM Glutamine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 24hrs
Other Notes
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