Mero-14
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 9100101, human lung, Spindle shaped cells
别名:
Mero14 Cells
生物来源
human lung
包装
tube of 5 μg 09100101-DNA-5UG
pkg of vial of cells 09100101-1VL
生长模式
Adherent
核型
Number of chromosomes 72-75
形态学
Spindle shaped cells
产品
Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
生化/生理作用
A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18
制备说明
Hams F10 + 15% FCS + 2mM Glutamine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 24hrs
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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