biological source
human lung
packaging
tube of 5 μg 09100101-DNA-5UG, pkg of vial of cells 09100101-1VL
growth mode
Adherent
karyotype
Number of chromosomes 72-75
morphology
Spindle shaped cells
products
Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
Biochem/physiol Actions
A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18
Preparation Note
Hams F10 + 15% FCS + 2mM Glutamine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 24hrs
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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