Mero-95

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

Mero-95 was derived from a pleural effusion

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11
D5S818: 11,14
D7S820: 7,9
THO1: 6
TPOX: 8,12
vWA: 18,19

Hams F10 + 15% FCS + 2mM Glutamine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 30 hours

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.