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Merck
CN

MKL-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 09111801, human skin. Loose floating aggregates with irregular outline and no central necrosis.

别名:

MKL1 Cells

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UNSPSC代码:
41106514
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生物来源

human skin

包装

tube of 5 μg 09111801-DNA-5UG
pkg of vial of cells 09111801-1VL

生长模式

Suspension

核型

Hyperdiploid

形态学

Loosely packed floating aggregates with irregular outline and no central necrosis

产品

MKL-1 is positive for CM2B4/MCV T AE1/AE2, CK20, EMA, CAM5.2, NSE and TTF-1. MKL-1 is negative for CK7, chromagen, synaptophysin, neurofilament, LCA, vimentin and CD99.

受体

Not specified

技术

cell culture | mammalian: suitable

相关疾病

metastasis

运输

dry ice

生化/生理作用

Human neuroendocrine skin carcinoma
MKL-1 was established from a nodal metastasis in a 26-year-old Caucasian male patient. MKL-1 cells contain a Merkel cell polyomavirus integrated into the genome. MKL-1 cells cause tumour formation at 4-6 weeks at subcutaneous sites in athymic male nude mice (BALB/c nu/nu).
STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 10,12
D5S818: 11,12
D7S820: 8,11
THO1: 9,9.3
TPOX: 8
vWA: 16,18

制备说明

RPMI 1640 + 2 mM Glutamine + 10% FBS
Seed at 3 x 105 cells/ml in 5% CO2; 37 °C Saturation density is approximately 106 cells/ml. Population doubling time is around 120 hours.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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