biological source
rat pancreas (islets)
packaging
tube of 5 μg 10020902-DNA-5UG, pkg of vial of cells 10020902-1VL
growth mode
Adherent
karyotype
Modal chromosome number 66 up to passage 50
morphology
Epitheloid
products
BRIN-BG7 is a stable, glucose-responsive insulin secreting beta cell line expressing insulin, glucokinase and IAPP as detected by immunocytochemistry. BRIN-BG7 cells express the GLUT2 glucose transporter.
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
-140 to -196°C
Biochem/physiol Actions
A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line
Not specified
Preparation Note
RPMI-1640 + 2 mM Glutamine + 10% FCS / FBS
Split sub-confluent cultures (70-80%) 1:8 to 1:10 , i.e., seeding at 2-4 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 20hrs. At confluence 105 cells/cm2 can be expected.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.