biological source
human colon
description
Colon carcinoma from human colon
packaging
tube of 5 μg 10092301-DNA-5UG, pkg of vial of cells 10092301-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
For complete product information, please see ECACC
Biochem/physiol Actions
Colorectal carcinoma from human colon
Human colorectal carcinoma
Preparation Note
Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS, Insulin 0.6?g/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Tony Burgess - Ludwig Institute for Cancer Research, Australia
法规信息
低风险生物材料
常规特殊物品
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