Mouse malignant mesothelioma

Mice (C57Black/6J strain female 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0156

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Optimal split ratio 1:16 - 1:32 (seeding density 0.6 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

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Depositor and originator: Richard Lake - University of Western Australia, Australia