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关于此项目
UNSPSC Code:
41106514
Biological source:
human esophagus (lung metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
Hypodiploid 39-41 chromosomes
Morphology:
Multicellular floating spheroids some with fibroblastoid morphology
biological source
human esophagus (lung metastasis)
packaging
tube of 5 μg 11012006-DNA-5UG, pkg of vial of cells 11012006-1VL
growth mode
Adherent
karyotype
Hypodiploid 39-41 chromosomes
morphology
Multicellular floating spheroids some with fibroblastoid morphology
products
P53 mutation c.164delC c.743G>A
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Application
Recommended culture medium: RPMI 1640 + 2mM L-Glutamine + 10% FBS
Biochem/physiol Actions
Barretts adenocarcinoma, adenocarcinoma of distal oesophagus
STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8.2,8.2
THO1: 6,9.3
TPOX: 8
vWA: 19,20
CSF1PO: 10,13
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8.2,8.2
THO1: 6,9.3
TPOX: 8
vWA: 19,20
Preparation Note
RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.