YTH 89.1

Monoclonal antibody human CD235a glycophorin A

Rat hybridoma producing antibody against human glycophorin A CD235a. Cambridge Enterprise Reference number AB66:www.enterprise.cam.ac.uk.

Human peripheral blood mononuclear cells

Not specified

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.

Maintain cultures between 3-9 x 10⁵ cells/ml. 5% CO₂; 37 °C. Saturation density 9 x 10⁵/ml

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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.