LIM1899 cell line human
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 12062001, human colon, Epithelial; cells are small rounded and grow in clusters
别名:
LIM 1899, LIM-1899
生物来源
human colon
包装
tube of 5 μg 12062001-DNA-5UG
pkg of vial of cells 12062001-1VL
生长模式
adherent
形态学
Epithelial; cells are small rounded and grow in clusters
运输
dry ice
储存温度
−196°C
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生化/生理作用
Columnar cell adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0163.
制备说明
Optimal split ratio 1:8 (seeding density 1 x 104 cells/cm2). Cells incubated at 37°C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM1899 requires the growth medium to be changed 3 times each week.
RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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