biological source
human liver (metastasis)
packaging
tube of 5 μg 12062002-DNA-5UG, pkg of vial of cells 12062002-1VL
growth mode
adherent
morphology
epithelial
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human liver metastasis, moderately differentiated sclerosing adenocarcinoma. Cell Bank Australia reference number: CBA0164.
Preparation Note
Optimal split ratio 1:4-1:6 (seeding density 0.8 x 104 cells/cm2). Cells incubated at 37°C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5-10 min.
RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10μM
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.